Semaglutide

What Is Semaglutide?

Semaglutide is a 31-amino-acid analog of human glucagon-like peptide-1 and one of the most widely studied selective GLP-1 receptor agonists. Its molecular formula is C₁₈₇H₂₉₁N₄₅O₅₉. Two design changes define the molecule: an α-aminoisobutyric acid substitution at position 8, which resists cleavage by the enzyme DPP-4, and a C18 fatty diacid joined to lysine 26 through a spacer, which drives reversible albumin binding.

Those modifications make semaglutide a textbook case of peptide half-life engineering, and laboratories use it well beyond incretin biology. Common applications include GLP-1 receptor binding and activation assays, receptor-activation assay models, head-to-head comparison with dual and triple agonists, and chromatographic method development where a well-characterized acylated peptide is needed as a standard. V8 Peptides supplies semaglutide as a 10 mg lyophilized powder.

Semaglutide Specifications

Reconstitution & Storage for Research

Semaglutide ships dry because the freeze-dried state is far more stable than a solution. Before use it is dissolved in a suitable laboratory diluent, and the volume chosen sets the stock concentration.

Concentration math

• Adding 2 mL of diluent to a 10 mg vial gives 5 mg/mL.
• Adding 1 mL of diluent to a 10 mg vial gives 10 mg/mL.
• Adding 2.5 mL of diluent to a 10 mg vial gives 4 mg/mL.
• At 5 mg/mL, every 0.2 mL of stock contains 1 mg of semaglutide.

The general rule is concentration (mg/mL) = vial content (mg) ÷ diluent volume (mL). Our calculator applies it for you and converts between milligrams and micrograms. Add the liquid slowly along the vial wall, give the cake a minute to wet, and swirl until no particles remain. Acylated peptides can foam, so do not shake.

Storage conditions

Unopened vials belong in a refrigerator at 2–8 °C; for long-term storage use −20 °C. Keep them dry, dark and away from heat sources. Reconstituted material should stay refrigerated, and dividing the stock into aliquots prevents the repeated freeze-thaw cycling that degrades peptides. See semaglutide storage and stability for a fuller discussion of temperature and light.

Quality & Testing

V8 Peptides releases semaglutide only after HPLC confirms the lot at ≥98% purity. A certificate of analysis is issued for each batch and published where anyone can read it, which lets a purchasing lab verify the material against its own acceptance criteria.

• HPLC purity result for the lot
• Identity and sequence confirmation
• Batch number and test date for traceability
• Supplied form and storage guidance

Certificates are posted on our COA page. If you are comparing vendors, semaglutide purity testing explains which figures on the report matter and why.

Related Lab Guides

• What is sema? The semaglutide research peptide explained
• Semaglutide reconstitution and handling
• Semaglutide storage and stability
• Semaglutide mechanism of action
• Semaglutide vs. tirzepatide
• Where to buy semaglutide for research
• Bacteriostatic water explained

For laboratory research use only; not for human consumption.