Tirzepatide

What Is Tirzepatide?

Tirzepatide (LY3298176) is a 39-residue synthetic peptide built on the native GIP sequence and engineered to engage two incretin receptors: the GIP receptor and the GLP-1 receptor. Because one molecule drives both pathways, it is often described in the literature as a dual agonist, and it has become a standard tool compound for laboratories that study incretin receptor signaling, biased agonism and glucose-regulation models.

The molecule (molecular formula C₂₂₅H₃₄₈N₄₈O₆₈) carries a C20 fatty diacid attached through a linker at a lysine side chain. This lipidation promotes albumin binding and is a well-characterized example of half-life engineering, which is why tirzepatide also turns up in formulation, stability and analytical-chemistry projects. In the lab it is used for receptor-binding experiments, cAMP accumulation assays, comparison against single-receptor GLP-1 agonists, and as a reference standard during method development. V8 Peptides supplies it as a 30 mg lyophilized vial.

Tirzepatide Specifications

Reconstitution & Storage for Research

A 30 mg vial holds more peptide than most single-compound vials, so the diluent volume you choose has a large effect on the working concentration. Decide on the stock strength your assay needs first, then work backward to the volume.

Concentration math

• Adding 3 mL of diluent to a 30 mg vial gives 10 mg/mL.
• Adding 2 mL of diluent to a 30 mg vial gives 15 mg/mL.
• Adding 1.5 mL of diluent to a 30 mg vial gives 20 mg/mL.
• Diluting 0.1 mL of a 10 mg/mL stock into 0.9 mL of buffer gives a 1 mg/mL working solution.

The V8 calculator handles these conversions for any volume, and the calculator guide explains each input. When adding diluent, direct the stream against the glass instead of onto the lyophilized cake, then roll or swirl the vial until the solution is clear. Avoid vortexing.

Storage conditions

Store sealed vials in the refrigerator at 2–8 °C, or move them to a −20 °C freezer for long-term holding. Heat, moisture and light all accelerate peptide degradation, so keep vials in their box and away from the freezer door. After reconstitution, keep the solution at 2–8 °C and aliquot it to limit freeze-thaw exposure. More detail is in our tirzepatide storage and stability article.

Quality & Testing

Purity claims are only useful when a lab can check them. Each tirzepatide batch is tested by HPLC against a ≥98% release specification, and the resulting certificate of analysis is tied to the lot number on the vial label.

• Measured HPLC purity for that batch
• Compound identity and sequence confirmation
• Lot number and date of analysis
• Form and recommended storage

You can open the current certificate from the COA library at any time. For background on how the analysis is run and how to read the report, see tirzepatide purity testing.

Related Lab Guides

• Tirzepatide reconstitution and handling
• Tirzepatide storage and stability
• Tirzepatide mechanism of action
• Semaglutide vs. tirzepatide: research comparison
• Where to buy tirzepatide: what to verify
• Tirzepatide chemical structure and synthesis

For laboratory research use only; not for human consumption.